Journal: PLoS ONE

Article Title: Critical Epitopes in the Nucleocapsid Protein of SFTS Virus Recognized by a Panel of SFTS Patients Derived Human Monoclonal Antibodies

doi: 10.1371/journal.pone.0038291

Figure Lengend Snippet: A) IFA analysis shows all selected MAbs are reactive to the viral and recombinant N protein. Vero: Vero cells infected by SFTSV strain HB29; Sf9: Sf9 cells expressing recombinant N protein with the baculovirus system; 293T: 293T cells transiently expressing the recombinant N protein. B) Western blot assay of human and mouse MAbs. Most MAbs represented a positive N protein band at 27 KDa, while MAbs H4B9, H4C8, H4E11 and H2H9 showed negative result. R. poly: rabbit polyclonal antibody immunized with the SFTSV N protein.

Article Snippet: Rabbit polyclonal and mouse monoclonal antibodies against N protein were produced commercially by AbMax Biotechnology company (China).

Techniques: Recombinant, Infection, Expressing, Western Blot

Journal: British journal of pharmacology

Article Title: Neuroprotection induced by dexpramipexole delays disease progression in a mouse model of progressive multiple sclerosis.

doi: 10.1111/bph.15058

Figure Lengend Snippet: FIGURE 4 Effects of dexpramipexole on innate immunity in progressive experimental autoimmune encephalomyelitis (PEAE) non-obese diabetic (NOD) mice. Representative confocal images and quantitation of immunostaining of (a) and (b) astrocytes (GFAP) and (c) and (d) microglia (IBA1) in spinal cord sections of MOG35-55-immunized NOD mice at Day 140 post immunization treated or not with dexpramipexole. In (b) and (d), each column is the mean ± SEM of five animals per group, six sections per animal (Kruskal–Wallis and post hoc Dunn's test). *P < 0.05 versus control (Crl)

Article Snippet: One hour later, sections were incubated overnight at 4 C with a rabbit polyclonal anti ionized calcium-binding adapter molecule 1 (IBA1) (1:300, Wako Cat# 013-26471, RRID: AB_2687911) for microglia, or with a mouse polyclonal antibodies against glial fibrillary acidic protein (GFAP) (1:500, Cell Signaling Technology Cat# 12389, RRID:AB_2631098).

Techniques: Quantitation Assay, Immunostaining, Control